Inhibition of DNA-dependent DNA-polymerase by ethidium bromide and 3,8-diamino-6-ethyl-5-methylphenanthridinium bromide
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منابع مشابه
The EcoRI restriction endonuclease, covalently closed DNA and ethidium bromide.
The reactions of the EcoRI restriction endonuclease on the covalently closed DNA of plasmid pMB9 were studied in the presence of ethidium bromide. At the concentrations of ethidium bromide tested, which covered the range over which the DNA is changed from negatively to positively supercoiled, the dye caused no alteration to the rate at which this enzyme cleaved the covalently closed DNA to yiel...
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A salt-induced cooperative conformational transition of a synthetic DNA, poly(dG-dC), is reversed by addition of ethidium bromide. Binding of the dye at high salt concentrations is highly cooperative. Circular dichroism spectra of the complex and the kinetic data support a model for this cooperative binding that is formally equivalent to the "allosteric" one proposed for oligomeric proteins by ...
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Eucaryotic cells contain two complete genetic systems, one based on nuclear DNA and the other on cytoplasmic DNA . The study of these systems has been greatly facilitated by the existence of specific inhibitors . One such inhibitor is the trypanocidal drug, ethidium bromide (Short, Peak, and Watkins, 1953) . Application of the drug to trypanosomes in vitro causes alterations in the structure of...
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The regulation of mitochondrial mass and DNA content involves a complex interaction between mitochondrial DNA replication machinery, functional components of the electron transport chain, selective clearance of mitochondria, and nuclear gene expression. In order to gain insight into cellular responses to mitochondrial stress, we treated human diploid fibroblasts with ethidium bromide at concent...
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The synthesis of mitochondrial DNA (mDNA) in HeLa cells is selectively inhibited by relatively low concentrations of ethidium bromide. After exposure of cells to strongly inhibitory concentrations of the drug, the apparent superhelix density of mDNA is rapidly increased, as judged by its buoyant density in CsCl in the presence of ethidium bromide. Mitochondrial DNA synthesized in the presence o...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1974
ISSN: 0014-5793
DOI: 10.1016/0014-5793(74)80498-9